Expression of RBBP4 and BCL-2 in gastric carcinoma and their clinicopathological significance / 临床与实验病理学杂志

ABSTRACT

Purpose To investigate the expression and significance of RBBP4 and BCL-2 in gastric carcinoma and to explore the relationship between their expression and clinicopathologic characteristics.Methods Immunohistochemistry was used to detect RBBP4 and BCL-2 proteins' expression in the 72 tumor tissues and para-cancer tissues.The association of RBBP4 expression with the clinicopathologic features and the expression correlation between RBBP4 and BCL-2 were analyzed.Real-time PCR was used to detect the expression of RBBP4 mRNA in the 20 tumor tissues and corresponding para-cancer tissues.Results Immunohistochemical results showed that 40 cases of cancer tissues exhibited RBBP4 positive expression,with a positive rate of 55.6％,and 36 cases showed BCL-2 positive expression (50％ positive rate),which were significantly higher than that in para-cancer mucosa (13.9％,16.7％).The over-expression of RBBP4 and BCL-2 was correlated with tumor differentiation,invasive depth and lymphatic metastasis in gastric carcinoma (P＜ 0.05).The expression of RBBP4 was positively correlated with BCL-2 (r =0.559,P ＜ 0.05).Real-time PCR results showed that the relative expression levels of RBBP4 mRNA in gastric carcinoma tissues and para-cancer tissues were 9.988 ± 1.948 and 1.458 ± 0.489 respectively,and the expression in gastric carcinoma was significantly up-regulated as compared with para-cancer tissues.Conclusions The expression of RBBP4 is up-regulated in gastric carcinoma,which may be involved in the development,invasion and metastasis of gastric carcinoma.RBBP4 might become a new diagnostic and prognostic marker for gastric carcinoma.