Anti-inflammation effects of psoralen and angelicin on human periodontal ligament cells in vitro / 上海交通大学学报（医学版）

ABSTRACT

Objective·To investigate the effects of psoralen and angelicin on inflammation cytokine expression of human periodontal ligament cells (hPDLCs).Methods· hPDLCs were primarily cultured using tissue explant method.Effects ofpsoralen and angelicin on the cell viability were tested by CCK-8 assay,hPDLCs were stimulated by Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) after treatment with different concentrations of psoralen and angelicin for 2 h.mRNA expression of IL-1β and IL-8 were determined by real-time PCR.Enzyme-linked immunosorbent assay (ELISA) was used to measure the secretion of IL-1β and IL-8.Results · hPDLCs were cultured successfully by tissue explant method.Psoralen and angelicin (≤ 12.5 μg/mL) did not show significant effects on the cell viability of hPDLCs.Pg-LPS markedly elevated the mRNA expression of IL-1β and IL-8,which could be attenuated by psoralen and angelicin in a dose-dependent manner.Likewise,the up-regulated protein secretion of IL-1β and IL-8 could be significantly blocked by psoralen and angelicin.Conclusion · Psoralen and angelicin could attenuate the inflammatory response of hPDLCs induced by Pg-LPS,Therefore,psoralen and angelicin may act as natural agents to prevent and treat periodontitis.