Effect of recombinant adiponectin on pregnancy outcome in spontaneous abortion model mice / 上海交通大学学报（医学版）

ABSTRACT

Objective·To investigate the effect of recombinant adiponectin on the pregnancy outcome and the balance of Th 17/regulatory T cell (Treg) in spontaneous abortion murine model.Methods· CBA/J(♀)×DBA/2(♂) mating was used as a spontaneous abortion model,while CBA/J(♀)×BALB/c(♂) mating as a normal pregnancy model.CBA/J female mice were intraperitoneal injected with 10 μg/(kg·d) recombinant adiponectin for 9 d from day 5 of gestation.The control group received equal volumes of PBS each day.On day 14 of gestation,the mice were killed.The embryo resorption rate was calculated.The levels of serum adiponectin,IL-17 and TGF-β were detected by ELISA.Spleen lymphocytes were isolated.The ratios of Thl 7/Treg were counted by flow cytometry(FCM).Results· Recombinant adiponectin administration had no obvious effect on the embryo resorption rate of the normal pregnancy model,while it significantly reduced the embryo resorption rate from 32.2％ to 13.3％ (P=0.039) of the spontaneous abortion model.ELISA showed that recombinant adiponectin increased the serum adiponectin and TGF-β levels,and reduced the IL-17 level of the spontaneous abortion model (P＜ 0.01).FCM showed that adiponectin administraion could reduce the ratio of Th 17/CD4+ T cells,increase the ratio of Treg/CD4+ T cells and reverse the ratio of Th 17/Treg in abortion mice.Conclusion · Intraperitoneal administration of recombinant adiponectin in early gestation can improve pregnancy outcome in a murine model of abortion maybe through regulating the balance of Th 17/Treg.