Influence of high glucose on PRKAA1 expression and proliferation of trophoblast cells / 上海交通大学学报（医学版）

ABSTRACT

Objective·To explore the expression level of protein kinase AMP-activated catalytic subunit α1 (PRKAA1) in placental tissues of gestational diabetes mellitus(GDM)women,and the influence of high glucose(HG)on PRKAA1 expression and proliferation viability of trophoblast cells in vitro. Methods·The placental samples of GDM women (n=19) and normal pregnant women (n=20) of the corresponding period were collected. Real-time qPCR and Western blotting assay were used to detect the mRNA and protein levels of PRKAA1 in these biopsies, respectively. Trophoblast cells were treated by HG in vitro and then expression level of PRKAA1 was tested.CCK8 assay was used to detect proliferation viability of the cells treated by HG medium or inhibitor of PRKAA1, dorsomorphin. Results·Comparing to normal pregnant women, both mRNA and protein levels of PRKAA1 in placental tissues of GDM women significantly decreased (both P<0.05). HG treatment drastically downregulated expression of PRKAA1 in trophoblast cells in vitro(P<0.05).Both HG medium and dorsomorphin suppressed proliferation viability of trophoblast cells(both P<0.05). Conclusion·Expression level of PRKAA1 is dampened in placental tissues of GDM women.HG suppresses proliferation viability of trophoblast cells probably via downregulating PRKAA1 level in vitro.