Drug Sensitivity and Resistance Gene of Carbapenem Resistant Enterobacteriaceae / 现代检验医学杂志

ABSTRACT

Objective To analyze the resistance genes of carbapenem resistant Enterohacteriaceae (CRE) from Zhongnan Hospital of Wuhan University,so as to provide evidence for CRE infection.Methods WHONET 5.6 software was used to analyze the resistance of carbapenem resistant Enterobacteriaceae isolated.Phenotype screening of 53 CRE strains taken with the modified Hodge test by three drug susceptibility slip to imipenem,meropenem and ertapenem,and analysed resistance gene analysis of 31 CRE strains phenotype screening.Results In 53 CRE strains,34 strains of Klebsiella pneumoniae,ac counting for 64.15 ％,12 strains of Escherichia coli,accounting for 22.64 ％,7 strains of Enterobacter cloacae accounted for 13.21％,and there were 31 strains of Hodge test positive strains,accounting for 58.49％ (31/53).The Hodge test results of three kinds of drug sensitive paper of imipenem,meropenem and ertapenem were consistent.In 31 strains of CRE,29 strains of blaKPC gone were amplified,accounting for 93.55 ％,9 strains of blaNDM gene.accounting for 29.03 ％,contained simaltaneous and two genes of blaKPC and blaNDM were 8 strains,accounting for 25.81 ％,the resistance genes of BlaIMP,blaOXA,and blaVIM were not amplified.Conclusion Resistance to carbapenems was mainly due to the production of KPC and NDM in Enterobacteriaceae of 31 strains.The CRE was detected in the Intensive Care Unit and Respiratory ward for mainly Klebsiella pneumoniae,and the main genotype was KPC.The CRE was detected in the Cadre ward mainly Escherich ia coli,and the drug resistant genotype was mainly a mixed type of KPC and NDM.