Effect of suppression of S6K1 by miR-195 oninvasion and metastasis of prostate cancer cell / 实用医学杂志

ABSTRACT

Objective To investigate the effects of inhibition S6K1 by miR-195 on proliferation,apoptosis,invasion and metastasis of prostate cancer cells.Method The prostate cancer cell line DU145 and LNCaP were used to establish cell lines with overexpression or suppression of miR-195.S6K1 mRNA expression was determined by the reverse transcription PCR (RT-PCR) assay.Cell proliferation was detected by MTT assay,cell apoptosis was detected by flow cytometry,cell invasion and metastasis was detected by transwell invasion test and cell scratch test,respectively.Target gene of miR-195 was identified by using dual luciferase reporter assay.Results Cell proliferation rate in miR-195 overexpression group was significantly lower than that in the control group.Cell apoptosis in miR-195 overexpression group was higher than that in the control group.The invasive cell numbers and scratch distance in miR-195 overexpression group were less than those in the control group,but the invasive cell numbers and scratch distance were increased in miR-195 knockdown group.S6K1 expression could be inhibited by miR-195,and S6K1 was identified as a target gene of miR-195.Conclusion miR-195 regulates proliferation,apoptosis,invasion and metastasis of prostate cancer cell by targeting S6K1.