Tetracycline hydrochloride induces the osteogenic differentiation of rat bone marrow mesenchymal stem cells / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 4605-4610, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-698280
ABSTRACT
BACKGROUND:
Tetracycline hydrochloride not only promotes the proliferation of bone marrow mesenchymal stem cells (BMSCs),but also has a strong bone activity.Studies on the osteogenic differentiation of BMSCs induced by tetracycline hydrochloride will provide a new cell source for bone tissue engineering.OBJECTIVE:
To observe the induction and differentiation activity of BMSCs into osteoblasts cultured by tetracycline hydrochloride in vitro.METHODS:
BMSCs were separated and expanded by whole bone marrow adherent method and identified by cell morphology and BMSCs cell markers CD34,CD45,CD90,CD29.Passage 3 BMSCs were collected and cultured with tetracycline hydrochloride (experimental group) or cultured in L-DMEM containing 10% fetal bovine serum (control group).Osteogenic induction and differentiation activity of BMSCs cultured by tetracycline hydrochloride were detected by alkaline phosphatase activity detection,collagen I and osteocalcin immunohistochemistry staining,alizarin red staining of mineralized calcium nodules,and detection of collagen I and osteocalcin gene expression by RT-PCR.RESULTS ANDCONCLUSION:
Tetracycline hydrochloride continuously promoted alkaline phosphatase activity in the BMSCs,significantly different from that in the control group.After 21 days of culture,positive expression of collagen I and osteocalcin proteins,red calcium nodules shown by alizarin red staining,and mRNA expression of collagen I and osteocalcin were observed in the experimental group,while the negative expression was found in the control group.In conclusion,tetracycline hydrochloride has a significant role to promote the osteogenic differentiation of BMSCs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Tissue Engineering Research
Ano de publicação:
2017
Tipo de documento:
Artigo
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