Effect of adiponectin in the treatment of nephrotic syndrome rats undergoing adipose stem cell transplantation / 中国组织工程研究

ABSTRACT

BACKGROUND: Adiponectin can regulate glucose and lipid metabolism in the body and protect body tissues and organs. Therefore, we combine it with stem cell transplantation for the treatment of nephrotic syndrome. OBJECTIVE: To investigate the therapeutic effect of adiponectin combined with adipose stem cell transplantation in the rats with nephrotic syndrome. METHODS:Rat adipose stem cells were resuscitated and cultured in vitro,and labeled using CD-Dil prior to the transplantation.Eighty Sprague-Dawley rats were randomly assigned into a normal control group, a model group, an adipose stem cell transplantation group, and a combined group (adiponectin and adipose stem cell transplantation), with 20 rats in each group. Rats in the normal control group received no treatment. The remaining rats in the latter three groups were given a single intravenous injection of adriamycin (6 mg/kg) to prepare animal models of nephrotic syndrome, followed by a tail vein injection of normal saline, 2×106/L adipose stem cell suspension, and 1 μg/kg adiponectin plus 2×106/L adipose stem cell suspension, respectively. Administration of adiponectin in the combined group was given once a day, for 3 consecutive days. RESULTS AND CONCLUSION: (1) Compared with the normal control group, higher levels of 24-hour urinary protein, blood cholesterol and urea nitrogen as well as lower serum albumin level were observed in the remaining three groups at 28 days after modeling. Compared with the model group, the levels of 24-hour urinary protein, blood cholesterol and urea nitrogen in the adipose stem cell transplantation group were decreased, while the level of serum albumin was increased. Compared with the adipose stem cell transplantation group, the levels of 24-hour urinary protein, blood cholesterol and urea nitrogen in the combined group were decreased, while the level of serum albumin was increased. (2) At 28 days after modeling, severest renal damage appeared in the model group, while the damage was reduced in the adipose stem cell transplantation group and considerably relieved in the combined group accompanied by improved inflammation and edema. (3) The number of CM-Dil positive cells in renal tissues was highest in the combined group followed by the adipose stem cell transplantation group, but no positive cells were detected in the normal control and model groups. Our experimental findings reveal that adipose-derived stem cell transplantation has a certain therapeutic effect on nephrotic syndrome, and adiponectin can enhance this therapeutic effect, by improving renal function and effectively inhibiting the pathological changes of renal tissues.