Influence of CaR-CaMCSE-H2S pathway on ischemia reperfusion related oxidative stress / 心血管康复医学杂志

ABSTRACT

Objective:To analyze influence of calcium sensitive receptor/cystathionine-γ-lyase hydrogen sulfide (CaR-CaM/CSE-H2S) pathway on ischemia reperfusion (I/R ) related oxidative stress .Methods:A total of 50 CSE gene knockout rats were selected as CSE gene knockout group ,another 50 normal rats were simultaneously enrolled as normal control group .Myocardial ischemia reperfusion injury (MIRI ) model was established in only CSE gene knockout group .Plasma total antioxidant capacity ,levels of reactive oxygen species (ROS) ,superoxide dismutase (SOD ) and malonyl diadehyde (MDA ) , expressions of cell apoptosis indexes (Bax , Bcl-2 , Caspase-3 mRNA , Caspase-8 mRNA ,Caspase-9 mRNA) ,calcium sensitive receptor (CaR) expression ,CSE activity and H2S content were measured and compared between two groups .Results: Compared with normal control group ,there were sig-nificant reductions in plasma total antioxidant capacity [(11.78 ± 1.14) U/ml vs.(10.20 ± 1.25) U/ml] ,SOD level [ (772.79 ± 5.21) nmol/L vs.(558.94 ± 5.36) nmol/L] ,CaR expression [ (2.85 ± 1.01)% vs.(1.04 ± 0.33)% ] , CSE activity [ (18.30 ± 1.70) nmol·L-1·min-1·g prot vs.(11.50 ± 1.50) nmol·L-1·min-1·g prot] and H2 S content [ (48.55 ± 1.35) μmol/g prot vs .(30.59 ± 1.41) μmol/g prot] ,and significant rise in levels of ROS [ (90.48 ± 1.42) U/ml vs.(99.74 ± 1.45) U/ml] and MDA [ (4.33 ± 1.15) U/ml vs.(4.98 ± 1.12) U/ml] ,ex-pressions of Bax [ (0.25 ± 0.05) vs.(0.47 ± 0.23)] ,Bcl-2 [ (0.18 ± 0.02) vs.(0.35 ± 0.05)] ,Caspse-3 mRNA [ (0.16 ± 0.04) vs.(0.25 ± 0.05)] ,Caspse-8 mRNA [ (0.20 ± 0.10) vs.(0.44 ± 0.15)] and Caspse-9 mRNA [(0.05 ± 0.01) vs.(0.15 ± 0.05)] in CSE gene knockout group ,P＜0.01 all.Conclusion:During I/R ,coronary en-docardial CaR expression down regulates ,CSE activity reduces and endogenous H2S generation decreases ,which ag-gravate oxidative stress injury caused by I/R .