Application of erlotinib based targeting fluorescent probe in the detection of lung cancer / 新乡医学院学报

ABSTRACT

Objective To explore the application of erlotinib based targeting fluorescent probe in the detection of lung cancer.Methods The erlotinib based targeting fluorescent probe was prepared.The lung cancer cells of A-549 were selected as experimental group,and cervical cancer cells of CaSki,SiHa and C33-A were selected as control group.The A-549,CaSki,SiHa and C33-A cells were identified by 0.1 × 10-6,1 × 10-6,10 × 10-6 mol · L-1 fluorescent probe;the identification ability of erlotinib based targeting fluorescent probe on cells in the two groups was observed under the inverted fluorescence microscope.Results When the concentration of fluorescent probe was 10 × 10-6 mol · L-1,the strong fluorescence signal was observed in A-549 and CaSki cells,but the fluorescence signal was not observed almost in SiHa and C33-A cells.When the concentration of fluorescent probe was 1 × 10-6 mol · L-1,the strong fluorescence signal was observed in A-549 cells;the weak fluorescence signal was observed in C33A cells;the fluorescence signal was not observed almost in SiHa and C33-A cells.When the concentration of fluorescent probe was 0.1 × 10-6 mol · L-1,the strong fluorescence signal was observed in A-549 cells;the fluorescence signal was not observed almost in CaSki,SiHa and C33-A cells.Conclusion Erlotinib based targeting fluorescent probe can specifically recognize lung cancer A-549 cells.