Expression of Caveolin-1 in Dorsal Root Ganglia and Spinal Dorsal Horn in the Development of Chronic Pain / 医学研究杂志

ABSTRACT

Objective To observe variation of Caveolin-1 expression in dorsal root ganglia and spinal dorsal horn in chronic neuropathic pain of rats.Methods Experimental animals of 56 SD rats were used for this study,and 28 rats for control.They were divided into 7 groups and their L5 nerves were ligated to produce the neuropathic pain model.After 1,3,5,7,10,14,21d of surgery,the mechanical shrinkage foot threshold and hot shrinkage foot incubation period were detected.Neuropathic pain models were confirmed to make successfully compared with the control group.Rats in the surgery group and the control group rats were all put to death on the 22d.The total protein of L5 spinal cord and dorsal root ganglia was extracted from the same side or the opposite side.The Caveolin-1 expression of different groups and sides was detected by the Western blot method in dorsal root ganglia and spinal dorsal horn in chronic neuropathic pain of rats.Results After 5,7,10,14,21d of L5 ligation,the expressions of Caveolin-1 were increased by 40.4％ ± 3.67％,126.9％ ± 5.46％,159.7％ ±4.89％,119.1％ ±5.77％ and 91.4％ ±5.31％ in the same sides.The difference was significant on 10d(P ＜0.01)after the surgery.After 5d,7 d,10d,14d,21 d of L5 ligation,the expressions of Caveolin-1 were increased by 33.3％ ± 4.89％,152.8％ ±3.56％,142.1％ ±5.43％,103.2％ ± 6.21％,175.6％ ±4.81％ in the opposite sides.It had statistic difference on 10d,14d,21d (P ＜ 0.05).There were no statistic differences among control groups.Conclusion The Neuropathic chronic pain can increase the Caveolin-1 expressions on the same side of dorsal root ganglia and spinal dorsal horn.