Effects of Different Dose 60Co Irradiation on Content of Isoimperatorin in Fengshi Bitong Capsule / 医药导报

ABSTRACT

Objective To establish a method for isoimperatorin in Fengshi Bitong capsule and to study the effects of different doses of 60Co irradiation on the content of isoimperatorin. Methods The Agilent C18 column(4.6 mm×150 mm, 5μm) was used with the mobile phase consisted of acetonitrile -water (4159) ;The flow rate was 1.0 mL?min-1 ,the detection wavelength was at 310 nm,and the column temperature was at 30 ℃ . Results Isoimperatorin showed a good linear relationship within a range of 3.66896-183.448 μg? mL-1( r = 1.0000).The average rate of recovery was 96.42％, RSD = 2.61％.The content of isoimperatorin in Fengshi Bitong capsule did not change significantly when irradiated by 2, 5 and 10 kGy of 60Co. Conclusion The established methods can be used for isoimperatorin determination in Fengshi Bitong capsule with high specificity,accuration and feasibility.The influence was not significant when the irradiation dose was less than 10 kGy.