Effect of miR-153-targeted PRDM2 on invasion and migration abilities of bladder cancer cells by JAK/STAT signaling pathway / 中国病理生理杂志

ABSTRACT

AIM:To investigate the effect of microRNA(miR)-153-targeted positive regulatory domain zinc finger protein 2(PRDM2 )on invasion and migration abilities of bladder cancer cells through JAK /STAT signaling path-way.METHODS:The expression levels of miR-153 in bladder cancer tissues were detected by qPCR.The expression of PRDM2 in normal tissues and bladder cancer tissues was detected by immunohistochemistry.The effect of miR-153 on the transcriptional activity of PRDM2 was examined by dual-luciferase reporter assay system.The effect of miR-153 over-ex-pression on the invasion ability of bladder cancer RT 4 cells was detected by Transwell assay ,and the cell invasion ability was analyzed by scratch test.The protein levels of PRDM2,p-JAK2 and p-STAT3 were determined by Western blot.RE-SULTSThe expression of miR-153 was significantly lower in the bladder cancer tissues than that in normal tissues(P<0.05).PRDM2 was highly expressed in the bladder cancer tissue(P<0.05).The results of dual-luciferase reporter assay system showed that miR-153 regulated the expression of PRDM2.miR-153 over-expression significantly decreased the invasion and migration abilities of bladder cancer RT 4 cells.miR-153 over-expression also down-regulated the protein levels of p-JAK2 and p-STAT3(P<0.05).CONCLUSION:miR-153 targets PRDM2,and regulates the invasion and migra-tion abilities of bladder cancer cells by JAK/STAT signaling pathway.