Effects of hypoxia exposure for different time on structure and function of red blood cells in rats / 中国病理生理杂志

ABSTRACT

AIM:To investigate the effects of hypoxia exposure on the structure and function of erythrocytes in rats at different time.METHODS:Male SD rats(n=40)were randomly divided into 5 groups,normal control group,1-week hypoxia group ,2-week hypoxia group ,3-week hypoxia group and 4-week hypoxia group ,with 8 rats per group.The rats in hypoxia groups were placed in the simulated 5800 m of high altitude in a hypobaric chamber for different time.The values of detected blood ,erythrocyte deformation index ,erythrocyte osmotic fragility ,erythrocyte oxygen dissociation ,e-rythrocyte apoptosis and bone marrow biopsy were determined.RESULTS:Compared with normal control group ,the red blood cell count ,hemoglobin content ,mean corpuscular volume and mean corpuscular hemoglobin significantly increased(P<0.01).Eversion rate of phosphatidylserine of erythrocytes increased.Oxygen half-saturation of hemoglobin increased(P<0.05).Bone marrow erythroid proliferation increased.The erythrocyte deformation index and erythrocyte osmotic fra-gility decreased significantly(P<0.01).In addition,oxygen dissociation curves shifted to the right.CONCLUSION:In the early stage of hypoxia ,compared with normal control group ,the changes of erythrocyte structure and function increase the oxygen supply to the tissue and are conducive to adapting to the plateau.However ,with the extension of hypoxia ,ex-cessive erythrocytosis results in thrombosis ,microcirculation disturbance and aggravating tissue hypoxia.