Effects of propofol on biological characteristics of colorectal cancer cells / 中国病理生理杂志

ABSTRACT

AIM:To investigate the effect of propofol on the viability,invasion ability and apoptosis of colorec-tal cancer cells.METHODS:Propofol at 10,25,50 and 100 μmol/L was used to treat LoVo cells for 72 h,and propofol at 100 μmol/L was used to treat the LoVo cells for 12,24,48 and 72 h.The cell viability was measured by CCK-8 assay. The invasion ability of the LoVo cells treated with propofol at 100 μmol/L for 72 h was detected by Transwell assay.The cell cycle distribution and cell apoptotic rate were analyzed by flow cytometry.The protein levels of matrix metalloproteinase (MMP)-2,MMP-9,cleaved caspase-3,Notch1 and hairy and enhancer of split 1(Hes1)were determined by Western blot.RESULTS:Propofol inhibited LoVo cell viability.The cell invasion ability,S stage cells,and the protein levels of MMP-2,MMP-9,Notch1 and Hes1 in propofol group were significantly lower than those in control group,and the apoptotic rate,G0/G1cells and the protein level of cleaved caspase-3 were significantly higher than those in control group(P<0.01).CONCLUSION:Propofol inhibits the viability and invasion ability of colorectal cancer LoVo cells,blocks cell cy-cle and induces apoptosis.The mechanism is related to down-regulation of Notch1 signaling pathway.