Effect of HDAC1 silencing on apoptosis of squamous cell carcinoma of skin via regulating STAT3 signaling pathway / 中国病理生理杂志

ABSTRACT

AIM:To investigate the effect of histone deacetylase 1(HDAC1)silencing on apoptosis of squa-mous cell carcinoma of skin.METHODS:Skin squamous cell carcinoma A431 cells were transfected with HDAC1 small interfering RNA(HDAC1 siRNA)or small interfering RNA negative control(siRNA NC).The expression levels of HDAC1 in transfected cells were detected by RT-PCR and Western blot.The cell viability was measured by MTT assay, and the apoptosis was analyzed by flow cytometry.The protein levels of STAT3,p-STAT3 and cleaved caspase-3 were de-termined by Western blot.The inhibitor of STAT3 signaling pathway was used to treat the A 431 cells transfected with HDAC1 siRNA.The cell viability was detected by MTT assay,the apoptosis was analyzed by flow cytometry,and the pro-tein levels of STAT3,p-STAT3 and cleaved caspase-3 were determined by Western blot.RESULTS: HDAC1 siRNA in-hibited the expression of HDAC1 at mRNA and protein levels in the A431 cells.After interfering with the expression of HDAC1,the cell viability and the protein level of p-STAT3 in the cells decreased,while the apoptotic rate and the protein level of cleaved caspase-3 in the cells were increased.After treatment with the inhibitor of STAT3 pathway,the viability of A431 cells transfected with siRNA and the protein level of p-STAT3 decreased,while the apoptotic rate and the protein le-vel of cleaved caspase-3 in the cells were increased.CONCLUSION: Interference with HDAC1 expression may regulate the STAT3 signaling pathway to inhibit the viability of skin squamous cell carcinoma cells,thus promoting the apoptosis of squamous cell carcinoma of skin.