Effect of ZNF281 on proliferation of hepatocellular carcinoma cells / 中国病理生理杂志

ABSTRACT

AIM:To investigate the role of zinc finger protein 281(ZNF281)in the proliferation of hepatocel-lular carcinoma(HCC)cells.METHODS:The mRNA expression levels of ZNF281 in peripheral blood mononuclear cells from 80 cases of healthy people and 206 cases of HCC patients were determined by real-time PCR.Statistical analysis were used to illustrate the relationship between the mRNA expression levels of ZNF 281 in the peripheral blood mononuclear cells and the clinicopathologic parameters of HCC patients.Real-time PCR and Western blot were used to detect the mRNA and protein expression levels of ZNF281 in hepatoma cell lines and immortalized hepatocytes.The silencing of ZNF281 was conducted by transfection of small interfering RNA targeting ZNF281,and then the proliferation of HCC cells was analyzed by MTS assay.The DNA synthesis of HCC cells was tested by Cell-LightTMEdU Apollo?488 In Vitro Imaging Kit.The ability of colony formation of the HCC cells was measured by colony formation assay,and the ability of anchorage-indepen-dent growth was detected by soft agar test.RESULTS: The mRNA expression level of ZNF281 in the peripheral blood mononuclear cells from HCC patients was significantly increased compared with the healthy people,and the high expression level was positively correlated with tumor size,tumor stage and tumor vascular invasion.Concurrently,the expression level of ZNF281 in hepatoma cell lines was significantly higher than that in immortalized hepatocytes.More importantly,the si-lencing of ZNF281 inhibited the proliferation, DNA synthesis, colony formation and anchorage-independent growth of the HCC cells.CONCLUSION:ZNF281 promotes the proliferation of HCC cells.