Pathological observation and serum biochemical indexes of traumatic osteoarthritis rat / 中华地方病学杂志

ABSTRACT

Objective To observe the relationship between serum hydroxyproline,nitric oxide (NO) and cartilage oligomeric matrix protein (COMP) and severity of traumatic osteoarthritis in rats,so as to provide scientific basis for prevention and treatment of Kaschin Beck disease.Methods Thirty-six healthy six-week-old male Wistar rats were divided into 3 groups according to weight via the random digital table method,including control group,ligamentum cutting group,ligamentum cutting + exercise group,with 12 rats in each group.Except the control group,the other two groups of rats were treated by anterior cruciate ligament transaction (ACLT).After 7 days,the ligamentum cutting + exercise group was tested at a 6-animal-treadmills for 20 min per day for 4 weeks.At the tenth week after operation,the surgical unilateral knee joints were taken;under a biological optical microscope,the gross joint morphology of each group was compared,which was scored according to C.Colombo articular cartilage pathological evaluation parameter standard.Rat serum was separated,and the contents of hydroxyproline,NO and COMP were determined.Results There were statistically significant differences among the 3 groups in the pathological evaluation of knee cartilage in rats (F =38.80,P ＜ 0.01).The scores of pathological evaluation of ligamentum cutting group,ligamentum cutting + exercise group were significantly higher than that of the control group (14.82 ± 6.19,18.25 ± 5.71 vs 1.58 ± 0.99,P ＜ 0.01).There was no statistical significant difference in comparison of ligamentum cutting group and ligamentum cutting + exercise group (P ＞ 0.05),and there was no statistical significant difference in serum hydroxyproline content of the 3 groups (F =2.15,P ＞ 0.05).There were statistical significant differences in serum NO and COMP of the 3 groups (F =36.13,16.77,P ＜ 0.01).Conclusions Traumatic osteoarthritis is caused by combining ACLT with movement,and serum levels of NO and COMP are closely related to the severity of osteoarthritis.Detections of NO and COMP are introduced into the research field of Kaschin-Beck disease,which will provide experimental basis for monitoring and treatment of Kaschin-Beck disease.