Heterogeneous HBV mutants coexist in Korean hepatitis B patients
Experimental & Molecular Medicine
;
: 115-122, 1998.
Artigo
em Inglês
| WPRIM
| ID: wpr-70150
ABSTRACT
Although many hepatitis B virus (HBV) mutants have been found in all open reading frames since the precore defective mutant was initially reported, systematic investigations of diverse HBV mutant populations in hepatitis B patients have not been performed. Therefore, we examined whether heterogeneous mutant populations simultaneously exist in Korean hepatitis B patients. In order to detect hepatitis B virus mutants, we amplified a conserved core region and a surface antigen region of HBV DNA by PCR from sera of 27 Korean chronic hepatitis B patients, and then performed single strand conformational polymorphism analysis followed by DNA sequencing analysis. The results showed that heterogeneous HBV mutants in both regions were present in a single as well as in various hepatitis B patients. Sequence analysis revealed a defective interfering particle with missense mutation in the core region. We also found that two subtypes of adr and adw coexisted in a single patient. In addition, a point mutation causing a stop codon in the surface antigen region was observed. We are further analyzing the clinical implications of HBV mutants to identify their roles in the pathogenesis of chronic hepatic disorders induced by HBV.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Dados de Sequência Molecular
/
Sequência de Bases
/
Vírus da Hepatite B
/
Reação em Cadeia da Polimerase
/
Análise de Sequência de DNA
/
Sequência Conservada
/
Polimorfismo Conformacional de Fita Simples
/
Heterogeneidade Genética
/
Hepatite B Crônica
/
Antígenos de Superfície da Hepatite B
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
1998
Tipo de documento:
Artigo
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