Protective effects of oridonin on adriamycin-induced myocardial apoptosis through inhibiting autophagy / 局解手术学杂志

ABSTRACT

Objective To investigate the effects and mechanisms of oridonin on adrimycin-induced myocardial apoptosis. Methods Cells were divided into H9C2,adriamycin,oridonin(5 μM),oridonin(10 μM) and oridonin(20 μM) group.Cells were treated with adriamy-cin except H9C2 group and cells in oridonin(5,10,20 μM)group were treated with oridonin at the same time.The concentrations of superox-ide dismutase(SOD) and malondialdehyde(MDA) were detected and the cell proliferation was detected by CCK8 assay.Cell apoptosis was determined by flow cytometry.The expressions of autophagy-related proteins(Beclin1,P62 and LC3) were measured by western blot.And the expression of LC3 also detected by immunofluoresence.Results Compared with H9C2 group,the concentration of SOD decreased and MDA increased in adriamycin group;compared with adriamycin group,SOD increased but MDA decreased in oridonin(5,10,20 μM)group signifi-cantly.Meanwhile,cell proliferation was inhibited and apoptosis was induced in adriamycin group compared with H9C2 group;but cell prolif-eration rate was increased and apoptosis rate was decreased in oridonin(5,10,20 μM)group compared with adriamycin group.In addition,ad-riamycin up-regulated the protein level of Beclin1 and ratio of LC3Ⅱ/LC3Ⅰ,and inhibited the expression of P62;oridonin(5,10,20 μM) attenuated the effects of adriamycin on Beclin1,ratio of LC3Ⅱ/LC3Ⅰand P62 notably.Conclusion Oridonin alleviated adriamycin-indued myocardial apoptosis by inhibiting autophagy.