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Preparation of polyclonal antibody and establishment of chemiluminescence immunoassay for aldosterone / 中国免疫学杂志
Chinese Journal of Immunology ; (12): 65-70, 2018.
Artigo em Chinês | WPRIM | ID: wpr-702675
ABSTRACT

Objective:

The polyclonal antibody of aldosterone (ALD) for immunoassay was developed.And a chemiluminescence immunoassay (CLIA) for the determination of ALD in human blood was established.

Methods:

Aldosterone oxime was prepared by chemical modification and then conjugated with BSA to prepare immunogen.Rabbit anti ALD polyclonal antibody was prepared by immunizing rabbits with the ALD-BSA.The CLIA of ALD was performed using biotin streptavidin amplification system and competition method.

Results:

After identification,rabbit No.3 received the highest sensitivity to ALD antibody,and the 50% binding inhibition (IC50) value for ALD concentration was 268 pg/ml.The measuring range of CLIA method using the antibody was 62.5-2 000 pg/ml.The assay sensitivity was 23.7 pg/ml.The intra-and inter-assay coefficients of variation were 6.9%-9.5% and 8.5%-12.7%,respectively.Analytical recovery rate was in the range of 93.1%-104.1%.The correlation coefficient between measured and expected values were 0.996 after serial dilution.Compared with radioimmunoassay kit,the correlative equation was y =0.932x+4.596,the correlation coefficient was 0.948 (n =95).

Conclusion:

The result of methodological identification shows that it was in line with the basic requirements of clinical application.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Immunology Ano de publicação: 2018 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Immunology Ano de publicação: 2018 Tipo de documento: Artigo