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Interactions between induced T regulatory cells and B cells in inflammatory milieu in mice with collagen-induced arthritis / 中国免疫学杂志
Chinese Journal of Immunology ; (12): 815-819,825, 2018.
Artigo em Chinês | WPRIM | ID: wpr-702823
ABSTRACT

Objective:

To investigate the interactions between induced T regulatory cells ( iTregs ) and B cells in the inflammatory milieu in mice with collagen-induced arthritis(CIA).

Methods:

CD19+ cells were isolated from the spleen cells of normal DBA1/J(N-B) mice and CIA mice(CIA-B) on the 35th day after the first immunization with established arthritis. These B cells were used as antigen-presenting cells to observe their effects on the induction of Tregs. Tregs were induced with the classic method and co-cultured with CIA-B cells. CIA-B cell effects on iTreg proliferation and the expression of CTLA-4 on iTregs were explored. iTregs′ influence on the expressions of co-stimulators(CD80,CD86) and MHCⅡon B cells was studied and its mechanism was determined by the Transwell experiments.

Results:

CIA-B could induce more Treg production and proliferation. CIA-B could also promote the CTLA-4 expression on iTreg cell surface which worked through a cell-contact pathway, while iTregs could increase the expressions of co-stimulators(CD80,CD86) and MHCⅡby the same way.

Conclusion:

iTregs could show their immune suppressive function through the interactions with CIA-B cells in the inflammatory milieu in mice with CIA. These interactions work by a cell-contact pathway.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Immunology Ano de publicação: 2018 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Journal of Immunology Ano de publicação: 2018 Tipo de documento: Artigo