Preparation and effect evaluation of the adjuvant vaccine of enteropathogenic Escherichia coli in Tibetan piglets / 中国人兽共患病学报

ABSTRACT

To study the immune protection of the inactivated vaccine against the enteropathogenic E.coli in Tibetan pigs,the strains isolated from the dead pig was identified by biochemistry and PCR methods.After that,the biological adjuvant vaccine was prepared by following procession.Firstly,selected enteropathogenic E.coli strain was cultured.Then,we harvested the bacteria and inactived it to prepare the antigen.Finally,we added the recombined cholera toxin B subunit as the biological adjuvant,added the mannose in solution 3 ％-5％ (W/V),distributed in ampoule,and freeze-dried.The performances of the vaccine was evaluated by administration for the nine groups of KM mice in oral and intramuscular immuno strategies,respectively.Results demonstrated that the effect of intramuscular injection of low dose containing adjuvant group were better than those without adjuvant group.The oral group contained both high dose of adjuvant group and low dose effect of immune adjuvant group were better than that of high and low dose not containing adjuvant group,and high dose of immune effect was better than low dose immune effect.The antibody titers proved that immunization for 4 times was much better than those immunization for times less than that.The data showed the vaccine was high protection against Tibetan Pig enteropathogenic E.coli challenge,especially the high dose of adjuvant vaccine was 100％ protection rate against enteropathogenic E.coli when orally immunization for 4 times in mice.