The MAK16 Gene of Entamoeba histolytica and Its Identification in Isolates from Patients
The Korean Journal of Parasitology
;
: 429-433, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-70336
ABSTRACT
To identify sequences of Entamoeba histolytica associated with the development of amebic liver abscess (ALA) in hamsters, subtractive hybridization of cDNA from E. histolytica HM-1IMSS under 2 growth conditions was performed 1) cultured in axenic medium and 2) isolated from experimental ALA in hamsters. For this procedure, 6 sequences were obtained. Of these sequences, the mak16 gene was selected for amplification in 29 cultures of E. histolytica isolated from the feces of 10 patients with intestinal symptoms and 19 asymptomatic patients. Only 5 of the 10 isolates obtained from symptomatic patients developed ALA and amplified the mak16 gene, whereas the 19 isolates from asymptomatic patients did not amplify the mak16 gene nor did they develop ALA. Based on the results of Fisher's exact test (P<0.001), an association was inferred between the presence of the mak16 gene of E. histolytica and the ability to develop ALA in hamsters and with the patient's symptoms (P=0.02). The amplification of the mak16 gene suggests that it is an important gene in E. histolytica because it was present in the isolates from hamsters that developed liver damage.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Expressão Gênica
/
Genes de Protozoários
/
Fatores de Virulência
/
Entamoeba histolytica
/
Estudos de Associação Genética
/
Abscesso Hepático Amebiano
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2014
Tipo de documento:
Artigo
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