Effects of PPARγ on the cholesterol efflux of C57 mice peritoneal macrophage in inflammatory conditions / 中国现代普通外科进展

ABSTRACT

Objective:To explore the effects of PPARγ on the cholesterol efflux of C57 mice peritoneal macrophage.Methods:Firstly constructing C57 mice model under different metabolic situation including high-fat diet and acute inflammation then isolate and culture its peritoneal macrophage,observing the expressions of PPARγ and IκB-α,identify the character of macrophage cholesterol efflux in every group.Then pretreat the normol C57 mice peritoneal macrophage with PPARγ ligand ciglitazone and PPARγ antisense oligonucleotide,observing the effect to cholesterol efflux after simulated with LPS in vitro.Results:The level of mice serum lipids of high fat diet group was significantly higher than that of normal diet group.The results of Western-blotting showed that the expression of PPARγ protein in groups of HFD and stimulated by LPS were significantly higher than that of control group.The expression in groups stimulated by LPS was all lower significantly than in control grouph.The determination of cholesterol efflux showed that this function of macrophage with HFD was more enhanced than that of control group but was inhibited in group stimulated by LPS.To normol peritoneal macrophage pretreat with PPARγ antisense oligonucleotide and stimulated by LPS,the expression of PPARγ protein was lower than that of control group but the expression of IκB-α was depressed obviously.Conclusion:The PPARγ ligand ciglitazone can increase the cholesterol efflux of C57 mice peritoneal macrophage and weaken the inhibition stimulated by LPS.The PPARγ antisense oligonucleotide can depress it and aggravate the inhibition.