Role of TLR4 on vascular injury induced by lower limb ischemia reperfusion / 中国现代普通外科进展

ABSTRACT

Objective:To explore the role of TLR4 in the process of lower limb ischemia-reperfusion injury.Methods:Ischemia Reperfusion injury model:in this study,the model of lower limb ischemia-reperfusion was established by rubber band wound method,C57BL / 6J mice were randomly divided into model control group(IR) and ischemia-reperfusion group(IR);TLR4 experiment:The mice models of ischemia reperfusion were divided into wild type (WT) model group and TLR4 knock out (TLR4-/-) model group;The expressions of TLR4 and HMGB1,TNF-α and IL6 were detected by RT-PCR and immunohistochemistry.The apoptosis of vascular wall was detected by TUNEL.Results:The expression of TLR4,HMGB1,TNF-α,IL6 in the IR group was significantly higher than that in the control group (P＜0.01).After TLR4 knockout,the expression of inflammatory factors and apoptosis in IR group was significantly reduced (P＜0.01).Conclusion:TLR4 mediates inflammatory response and induces apoptosis in lower limb ischemia reperfusion injury in mice.