Effects of Aerobic Exercise and Polysaccharide Compounds Interventions on Gut Microbiota of the High Fat Diet Induced Obese Rats / 中国运动医学杂志

ABSTRACT

Objective To analyze and verify the effects of different intervention on the gut microbiota of high fat diet(HFD) induced obese rats.Methods Totally 70 male Sprague-Dawley rats were enrolled in this test.After 4 weeks of HFD feeding,40 HFD induced obese rats were randomly divided into an obese control group (n=10),an aerobic exercise group(n=10),a polysaccharide lavage group(n=10),and an aerobic exercise combined with polysaccharide lavage group (n=10).The moderate intensity treadmill training,polysaccharide lavage and their combinations were conducted as their group names indicated.The gut microbiota was analyzed using the 16S rDNA sequencing before and after the intervention.Results Compared with the control group,the ratio of ruminococcacea and ruminococcaceae bromii increased significantly,while that of turicibacteraceae,bifidobacterium and turicibacter reduced significantly in HFD induced obese rats.Compared with the obese control group,aerobic exercise increased the ratio of bifidobacteriaceae,akkemansia,bifidobacterium,akkermansia mucinphila and prevetella copri significantly,but reduced that of helicobacteraceae and ruminococcaceae significantly.Compared with the obese control group,the polysaccharide lavage increased the ratio of bifidobacteriaceae and turicibacteraceae,bifidobacterium,together with allobaculum and turicibacter significantly,but reduces that of desulforibrionaceae,helicobacteraceae and ruminococcaceae,as well as ruminococcaceae bromii significantly.Compared with the obese control group,significant increase was observed in the ratio of bifidobacteriaceae,allobaculum and blautia in the aerobic exercise combined with polysaccharide lavage group.Conclusions The ratio of obesity related bacteria such as ruminococcus bromii increases significantly in the HFD induced obese rats.All of the three different interventions can optimize the gut microbiota composition of the HFD induce obese rats effectively by increasing the ratio of beneficial bacteria.However,they have significant differences on the ratio of different bateria.