Simultaneous Determination of Eugenol and Thymol in Mummification Pastes by HPLC / 中国药房
China Pharmacy
;
(12): 4268-4271, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-704424
ABSTRACT
OBJECTIVE:
To establish a method for simultaneous determination of eugenol and thymol in mummification pastes.METHODS:
HPLC method was adopted.The determination was performed on Wonda Cract C18 column with mobile phase consisted of methanol-0.2% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was 274 nm,and column temperature was set at 25 ℃.The sample size was 10 μL.RESULTS:
The linear ranges of eugenol and thymol were 0.02-0.19 mg/mL (r=0.999 7) and 0.03-0.30 mg/mL (r=0.999 6).RSDs of precision,stability and reproducibility tests were all lower than 2.0%.The recoveries were 95.85%-99.84%(RSD=1.11%,n=9) and 95.81%-100.91%(RSD=1.59%,n=9).CONCLUSIONS:
The method is simple and accurate,and can be applied to simultaneous determination of eugenol and thymol in mummification pastes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2017
Tipo de documento:
Artigo
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