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Simultaneous Determination of Eugenol and Thymol in Mummification Pastes by HPLC / 中国药房
China Pharmacy ; (12): 4268-4271, 2017.
Artigo em Chinês | WPRIM | ID: wpr-704424
ABSTRACT

OBJECTIVE:

To establish a method for simultaneous determination of eugenol and thymol in mummification pastes.

METHODS:

HPLC method was adopted.The determination was performed on Wonda Cract C18 column with mobile phase consisted of methanol-0.2% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was 274 nm,and column temperature was set at 25 ℃.The sample size was 10 μL.

RESULTS:

The linear ranges of eugenol and thymol were 0.02-0.19 mg/mL (r=0.999 7) and 0.03-0.30 mg/mL (r=0.999 6).RSDs of precision,stability and reproducibility tests were all lower than 2.0%.The recoveries were 95.85%-99.84%(RSD=1.11%,n=9) and 95.81%-100.91%(RSD=1.59%,n=9).

CONCLUSIONS:

The method is simple and accurate,and can be applied to simultaneous determination of eugenol and thymol in mummification pastes.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacy Ano de publicação: 2017 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacy Ano de publicação: 2017 Tipo de documento: Artigo