Simultaneous Determination of 4 Active Components in Compound Xiaosuanzao Chewable Tablets by HPLC-ELSD / 中国药房
China Pharmacy
;
(12): 4275-4277, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-704426
ABSTRACT
OBJECTIVE:
To establish the method for the content determination of 4 active components in Compound xiaosuanzao chewable tablets.METHODS:
HPLC-ELSD method was adopted.The determination was performed on a Grace Brava C18-BDS column with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 1.0 mL/min.The column temperature was 25 ℃,and sample size was 20 μL.The drift tube temperature is 100 ℃,and the carrier gas flow rate is 2.9 L/min.RESULTS:
The linear ranges of betulic acid,betulinol,pachymic acid and glycyrrhizic acid were 44.50-890.0 μg/mL (r=0.999 3),20.28-405.6 μg/mL (r=0.999 7),20.50-656.0 μg/mL(r=0.999 7) and 10.50-336.0 μg/mL(r=0.999 6),respectively.RSDs of precision,stability and reproducibility were all lower than 3.0%.The recoveries were 99.44%-101.12% (RSD=0.57%,n=6),99.41%-100.39% (RSD=0.34%,n=6),99.31%-100.46% (RSD=0.51%,n=6),98.96%-101.19% (RSD=0.84%,n=6),respectively.CONCLUSIONS:
The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of 4 active components in Compound xiaosuanzao chewable tablets.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2017
Tipo de documento:
Artigo
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