Content Determination of 3 Components in Li'ermian Capsule by HPLC / 中国药房
China Pharmacy
;
(12): 62-64, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-704520
ABSTRACT
OBJECTIVE:
To establish a method for simultaneous determination of 3 components in Li'ermian capsule,including berberine hydrochloride,palmatine hydrochloride and cinnamaldehyde.METHODS:
HPLC method was adopted.The determination was performed on Agilent Zorbax SB-C18 with mobile phase consisted of acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution (23 ∶ 77,V/V) at the flow rate of 1.0 mL/min.The detection wavelengths were set at 345 nm (0-20 min,berberine hydrochloride,palmatine hydrochloride) and 290 nm (>20-30 min,cinnamaldehyde),and column temperature was 30 ℃.The sample size was 10 μL.RESULTS:
The linear ranges of berberine hydrochloride,palmatine hydrochloride and cinnamaldehyde were 0.036 80-0.736 0,0.016 76-0.335 2,0.004 140-0.082 80 μg (r≥0.999 5).The limits of detection were 0.110 4,0.050 3,0.124 2 ng,and the limits of quantitation were 0.368 0,0.167 6,0.414 0 ng,respectively.RSDs of precision,stability (12 h) and reproducibility tests were all lower than 2.0% (n=6).The recoveries were 96.16%-99.73% (RSD=0.99%-1.21%,n=6).CONCLUSIONS:
Established method is simple,reproducible and suitable for simultaneous determination of 3 components such as berberine hydrochloride in Li'ermian capsule.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2018
Tipo de documento:
Artigo
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