Study on HPLC Fingerprint of Miao Medicine Fufukang Spray / 中国药房
China Pharmacy
;
(12): 1529-1532, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-704837
ABSTRACT
OBJECTIVE:
To establish HPLC fingerprint of Miao medicine Fufukang spray.METHODS:
The determination was performed on Thermo Hypersil GOLD C18 column with mobile phase consisted of acetonitrile-0.2% glacial acetic acid (gradient elution) at the flow rate of 0.6 mL/min. The detection wavelength was set at 262 nm,and column temperature was 25 ℃. The sample size was 10 μL. Using 6′-hydroxyl-justicidin B as reference control,HPLC chromatograms of 10 batches of samples were drawn. Similarity evaluation system software(2004 A edition)of TCM fingerprint was used to evaluate the similarity and determine common peaks of 10 batches of samples. The established method was used to determine the contents of 6′-hydroxyl-justicidin B and justicidin B in 10 batches of samples.RESULTS:
There were 12 common peaks in HPLC fingerprints of 10 batches of samples with similarity>0.90. HPLC fingerprint showed a good consistency with the control fingerprint. The contents of 6′-hydroxyl-justicidin B and justicidin B in 10 batches of samples were 1.64-5.84,0.83-2.78 mg/100 mL,respectively.CONCLUSIONS:
The established method is simple,accurate,reliable and suitable for quality evaluation of Fufukang spray.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacy
Ano de publicação:
2018
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS