Effect of diabetes on CCl4-induced rats liver fibrosis / 中国药理学通报

ABSTRACT

Aim To observe the effect of diabetes on carbon tetrachloride (CCl4)-induced rats chemical liv-er injury and liver fibrosis by establishing diabetes mer-ged with liver fibrosis rat model (double model). Methods High fat feeding combined with streptozoto-cin (STZ) was used to induce diabetes rat model,and liver fibrosis rat model was induced by CCl4. HE stai-ning was used to observe the rat liver pathological changes, and Western blot and q-PCR were used to detect liver fibrosis related factor genes α-SMA and Collagen Ⅰ expression. Results Compared with con-trol group, diabetes group, liver fibrosis group and double model group all had different levels of liver damage,especially double model group. Rat liver tis-sues of α-SMA and CollagenⅠexpression from differ-ent model groups also increased, especially those from double model group, and significant differences were detected compared to diabetes and liver fibrosis group. Conclusions Diabetes can cause liver damage and in-crease the occurrence and development of CCl4-in-duced rats liver fibrosis, and the mechanism may be related to the formation and/or degradation of extracel-lular matrix.