Effects of Jiannao Yishou Capsule on Learning and Capillary Structure of Hippocampal in Alzheimer's Dis-ease Mice / 中国药师

ABSTRACT

Objective:To investigate the protective effects of Jiannao Yishou capsule(JYC) on hippocampal in Alzheimer's dis-ease (AD) mice. Methods:Male mice were randomly divided into normal control group,model control group,positive control group (donepezil hydrochloride tablets,0.001 g·kg-1),JYC high-dose (3.744 g·kg-1) and low-dose (0.936 g·kg-1) group. Morris water maze was used to test the learning and memory ability of mice. The hippocampal capillary structure was observed by light micros-copy and electron microscopy and the level of hippocampal Aβ was measured by enzyme-linked immunoassay.Results:Compared with the normal control group,the model control group learning and memory significantly reduced,and capillary endothelial cell mitochon-drial degeneration,nuclear condensation,broadening of the perinuclear space,overlapping,Aβ in hippocampal of AD mice were sig-nificantly reduced (P<0.05). Compared with the model control group,the JYC high-dose group learning and memory of mice im-proved significantly (P<0.05),and hippocampal capillary endothelial cell morphology improved significantly,and Aβ in Hippocam-pal of AD mice were significantly reduced(P<0.05). Conclusion:JYC can maintain normal morphological structure of hippocampus capillaries of AD mice,decrease Aβ responses and Improve learning and memory. JYC has protective effects on hippocampal tissues in AD mice.