Improvement of HPLC Content Determination for Esmolol Hydrochloride Injection / 中国药师
China Pharmacist
;
(12): 180-182, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-705485
ABSTRACT
Objective:
To improve the HPLC method for the determination of esmolol hydrochloride injection .Methods:
HPLC was used and the column was a Welch Xltimate C 18 column(250 mm ×4.6 mm, 5μm).The mobile phase was acetonitrile -methanol-phosphate buffer (152065), and the detection wavelength was 222 nm.The flow rate was 1.0 ml· min-1, the column temperature was 30℃, and the sample volume was 20μl.Results:
The concentration of esmolol hydrochloride showed a good linear relationship ( r=1.0000) with the peak area within the range of 12.26-196.16 μg· ml-1, and the average recovery was 101.2%(RSD=0.7%, n=9).Conclusion:
The method is simple, rapid, accurate and reproducible , which can show truer drug content when compared with the current standard , and is more suitable for the content determination of esmolol hydrochloride injection .
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2018
Tipo de documento:
Artigo
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