Determination of Three Components in Xylaria Nigripes Powder by HPLC / 中国药师
China Pharmacist
;
(12): 527-529, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-705579
ABSTRACT
Objective:
To establish a HPLC method for the simultaneous determination of adenosine,genistein and 5-hydroxymel-lein in Xylarin nigripes powder.Methods:
The chromatographic separation was carried out on a Pntulips@BP-C18column (250 mm× 4.6 mm,5 μm) at 35℃. The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution at a flow rate of 1.0 ml ·min-1,and the detection wavelength was 254 nm.Results:
The linear range of adenosine was 0.162-1.62 μg(r=0.999 2). The average recovery was 98.12% and the RSD was 1.32% (n=6). The linear range of genistein was 0.02-0.2 μg(r=0.999 9). The average recovery was 98.89% and the RSD was 1.10% (n =6). The linear range of 5-hydroxymellein was 0.022-0.22 μg(r =0.999 9). The average recovery was 99.87% and the RSD was 0.98% (n=6).Conclusion:
The method can be used to determine the contents of adenosine,genistein and 5-hydroxymellein,which provides the basis for the quality control and the further research of Xylaria nigripes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2018
Tipo de documento:
Artigo
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