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Study on the HPLC Fingerprint of Yiqing Granules / 中国药师
China Pharmacist ; (12): 836-839, 2018.
Artigo em Chinês | WPRIM | ID: wpr-705604
ABSTRACT

Objective:

To establish an HPLC fingerprint of Yiqing granules to provide reference for the effective quality control.

Methods:

The analysis was carried out on an analytical Agilent ZORBAX SB-C18(250 mm×4.6 mm,5 μm)column with gradient elution by acetonitrile(A)-0.2% phosphoric acid solution(adjusting pH to 3.0 with triethylamine,B)(0-5 min10% A→20%;5-20 min20% A→30% A;20-25 min30% A→45% A;25-35 min45% A;35-45 min45% A→80% A;45-50 min80% A)at the detection wavelength of 254 nm and the flow rate of 1.0 ml·min-1,and the column temperature was 35 ℃. Twelve batches of Yiqing granules were determined by HPLC and a common mode of fingerprint was established.

Results:

There were 22 common peaks in the fingerprints of the twelve batches of Yiqing granules,and seven of them were identified. The similarities of fingerprints of the twelve batches of Yiqing granules were over 0.990 suggesting good reproducibility.

Conclusion:

The developed method is accurate and feasible,and can be used for the quality control of Yiqing granules.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacist Ano de publicação: 2018 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: China Pharmacist Ano de publicação: 2018 Tipo de documento: Artigo