Determination of Six Active Ingredients in Gualoupi Injection by Quantitative Analysis of Multi-components by Single Marker / 中国药师
China Pharmacist
;
(12): 1779-1782, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-705704
ABSTRACT
Objective:
To develop a method of quantitative analysis of multi-components by single marker (QAMS) for six active ingredients in Gualoupi injection.Methods:
An HPLC method was used with a Waters Sunfire ODS C18column (250 mm×4. 6mm, 2. 5 μm), the mobile phase was methanol(A)-0. 2% glacial acetic acid solution(B) with gradient elution, the flow rate was 1. 0 ml· min-1,the detection wavelength was 254 nm,the column temperature was 30℃ and the injection volume was 20 μl. Using 3, 29-dibenzoyl rarounitriol as a reference, the relative correction factors among karounidiol, vanillic acid, adenosine, quercitrin and cynaro-side were detected by QAMS and their contents were calculated, and the results were compared with those of the external standard method.Results:
The differences were not statistically significant between the calculated values of karounidiol, vanillic acid, adeno-sine, quercitrin and cynaroside and those measured by the external standard method (P>0. 05).Conclusion:
QAMS can be used for the determination of 6 effective components in Gualoupi injection, and the result is accurate, simple and effective.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
China Pharmacist
Ano de publicação:
2018
Tipo de documento:
Artigo
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