Correlation of Virologic Property and Phylogenetic Analysis of Hantaan Viruses Isolated from Patients and Reservoirs in Korea
Journal of the Korean Society of Virology
; : 11-20, 1998.
Article
em En
| WPRIM
| ID: wpr-70607
Biblioteca responsável:
WPRO
ABSTRACT
Twenty isolates of Hantavirus were isolated from patients and reserovirs from 1988 to 1994 in Korea. Isolation rate was 1.9% (10/538) in patients, 6.2% (5/81) in Apodemus sp., 2.6% (1/38) in Rattus sp. and 0.6% (4/677) in bats. Reciprocal mean IFA titers ranged from 27.5 to 1,024 at the specimen collection. According to the growth rate and reaching peak titer of infectivity, the isolates were grouped as rapid, intermediate, and slow growing groups. All isolates were confirmed as Hantaan type by the nested RT-PCR on the Gl region of the M segment. Comparison of nucleotide sequence (Nt: 2101 - Nt: 2280) of the G2 region revealed that the sequence homology between Hantaan 76/118 virus and the isolates was more than 90%. Several nucleotide positions of the isolates showed high variation. The variation rate of patientisolates was about one-half when compared with that of rodentisolates. On the basis of phylogenetic analysis Hantaan viruses isolated were divided into two genogroups. These results indicate that Hantaan virus is highly dominant serotype in Korea and the virologic property and genogroup are not correlated.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Manejo de Espécimes
/
Sequência de Bases
/
Quirópteros
/
Orthohantavírus
/
Homologia de Sequência
/
Vírus Hantaan
/
Murinae
/
Genótipo
/
Coreia (Geográfico)
Limite:
Animals
/
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Journal of the Korean Society of Virology
Ano de publicação:
1998
Tipo de documento:
Article