Uptake Study on Apigenin in Caco-2 Cells / 中国中医药信息杂志
Chinese Journal of Information on Traditional Chinese Medicine
; (12): 74-78, 2018.
Article
em Zh
| WPRIM
| ID: wpr-706996
Biblioteca responsável:
WPRO
ABSTRACT
Objective To study the uptake property of apigenin in Caco-2 cells.Methods Caco-2 cell monolayer model was used to research the effects of apigenin on cell uptake in different times, concentrations, temperatures, pH values, and P-gp inhibitors. HPLC was used to determine the content of apigenin in cells.Results The linearity of apigenin concentration curve was found in good linear range of 0.33–80 μg/mL (r=0.9994). Uptake determination of apigenin in Caco-2 cells depended a certain of time, concentration and pH values, and P-gp inhibitors could not increase the apigenin uptake.Conclusion This method is simple and sensitive. P-gp is not involved in apigenin transport process, and uptake of apigenin in Caco-2 cells is mainly passive transported.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Information on Traditional Chinese Medicine
Ano de publicação:
2018
Tipo de documento:
Article