Evaluation of the potential diagnostic value of cytotoxic T lymphocyte-associated antigen-4 in differentiation of active and latent tuberculosis infection / 中华传染病杂志
Chinese Journal of Infectious Diseases
; (12): 397-400, 2018.
Article
em Zh
| WPRIM
| ID: wpr-707236
Biblioteca responsável:
WPRO
ABSTRACT
Objective To analyze the expressions of cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) in the peripheral blood of patients with active tuberculosis (ATB ) or latent tuberculosis infection (LTBI) ,and to evaluate its diagnostic value in differentiation of ATB and LTBI .Methods Forty-eight patients including 18 ATB cases and 30 LTBI cases were continuously enrolled from Wuxi No . 5 People′s Hospital and Huashan Hospital affiliated to Fudan University from January 2011 to March 2013 .Flow cytometry was applied to detect the CTLA-4 expression in CD4+CD25+ FoxP3+ T cells in the peripheral blood of the 48 subjects .CTLA-4 levels were compared using non-parametric Mann-Whitney U test .Results The median percentage of CTLA-4+ Treg in CD4+ CD25+ Foxp3+ Treg cells of ATB patients was 18 .95% (quantile range :13 .86% ,27 .73% ) ,and that in LTBI patients was 6 .67%(quantile range :5 .74% ,9 .59% ) ,which was statistically significant (U=18 .0 , P< 0 .01) .Receiver operating curve (ROC) based on the CTLA-4 expression indicated that the area under the curve was 0 .96 , with the optimum cut-off value of 13 .25% .Thus ,the sensitivity and specificity for the diagnosis of ATB were 86 .7% and 94 .4% ,respectively .Conclusion CTLA-4 has highly sensitivity and specificity for the differential diagnosis of ATB and LTBI whose interferon-gamma releasing assays are all positive ,which may also provide meaningful clue for the study of pathogenesis of ATB .
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Índice:
WPRIM
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Infectious Diseases
Ano de publicação:
2018
Tipo de documento:
Article