The application of ultrasound combined with molecular markers Galectin-3 ,c-Met ,HBME-1 ,CK19 expressions for lymph node metastasis and prognosis in thyroid carcinoma / 中华超声影像学杂志

ABSTRACT

Objective To explore the correlation between Galectin-3 ,c-Met , HBME-1 ,CK19 and lymph node metastasis of thyroid carcinoma ,and study the value of ultrasound combined with the markers in estimating prognosis . Methods From December 2015 to September 2016 ,46 patients with thyroid nodules (8 were benign and 38 were malignant) who undergone surgery in our hospital were screened out . Preoperative analysis of ultrasonic manifestations of cervical lymph nodes and the evaluation of lymph node had been done before surgery .Immunohistochemical test of Galectin-3 ,c-Met ,HBME-1 and CK19 by Maxvision one step method for postoperative paraffin wax block were performed . Pathology was the gold standard for the final determination of metastatic lymph nodes . Results Chi-square and prospective chi-square test results showed that ultrasound assessment of metastatic lymph nodes compared with the final pathological results were statistically significant ( P ＜ 0 .001) . The expressions of Galectin-3 and c-Met were statistically significant compared with the lymph node metastasis confirmed by pathology ( Galectin-3 :P ＝0 .005 ;c-Met : P ＜0 .001) . The expressions of HBME-1 and CK19 were not statistically significant ( HBME-1 : P ＝0 .162 ,CK19 : P ＝0 .154) . ROC curve results showed that the comparison of Galectin-3 and c-Met and ultrasound cut-off value had the highest diagnostic value ,the sensitivity was 92 .3% and the specificity was 80 .0% . Conclusions The expressions of Galectin-3 and c-Met are associated with lymph node metastasis of thyroid carcinoma ,but HBME-1 and CK19 are not . Ultrasound combined with the expression of the markers for lymph node metastasis is valuable in guiding the prognosis of patients .