Design and application of a batch-reactor microfluidic device for synthesis of 18F PET tracers / 中华核医学与分子影像杂志

ABSTRACT

Objective To develop an automated control system of batch-reactor microfluidic device for the synthesis of PET tracers and to use it for the preparation of 18F-fluorodeoxyglucose (FDG).Methods The 18F microreactor was composed of polydimethylsiloxane (PDMS) microfluidic chip and customized glass microvessel integrated with stainless capillary tube as heater or cooler.PDMS chip was fabricated by silkscreen printing technology.The hardware control of digital and analog quantity of synthesizer was completed by organic integration programmable logic controller (PLC),micro air valve,temperature sensor,com pressed air source,direct current stabilized voltage source and vacuum pumps.The interface was designed using Kingyiew software.Thin-layer chromatography (TLC) was applied to measure the 18F-labeling yield and the radiochemical purity of 18F-FDG.Kryptofix (K2.2.2) content,residual acetonitrile content,traits,a septic and bacterial endotoxins levels were also tested.Results The size of the microfluidic device was 10 cm× 10 cm×15 cm.The size of the automated control system was similar to the desktop chassis.The amount of precursor used in the single synthesis of 18F-FDG was 2.5 mg.The radiochemical purity of 18F-FDG was higher than 95％,the labeling yield was (92.4± 1.4) ％ and the 18 F-FDG yield was (35.6± 5.6) ％ (decay corrected).The 18F-FDG product was clear and colorless,and the pH value was 6.2±0.4.The K2.2.2 con tent was less than 50 μg/g.The residual acetonitrile content was (12.8±2.6) μg/g.Both aseptic and bacte rial endotoxins tests were negative.Conclusions A batch-mode microfluidic device is developed and successfully applied to prepare 18F-FDG.It has the advantages of high integration,small size,less consumption of labeling precursor and easy programming.