Anti-bacterial and anti-inflammatory effects of Qingre Huashi Formula on Helicobacter pylori-infected mice / 中成药

ABSTRACT

AIM To evaluate the bactericidal activity of Qingre Huashi Formula (Coptidis Rhizoma,Gardeniae Fructus,Taraxaci Herba,etc.) against Helicobacter pylori in infected mice and the effects on NF-κB p65,IL-1 β,IL-8 and TNF-α expression levels.METHODS Forty-four SPF KM mice were randomly divided into four groups,blank control,model control,Qingre Huashi Formula and triple therapy (metronidazole,clarithromycin and lansoprazole) groups.Except the blank control group,mice in another three groups were infected with H.pylori through short-term immunosuppression combined with oral infection.Administration lasted for four weeks.All mice were sacrificed.Rapid urease test (RUT) and histological staining were performed to identify the eradication of H.pylori.HE staining was applied to observing the H.pylori colonization degree and gastric mucosal inflammation.NF-κB p65,IL-1 β,IL-8 and TNF-α expression levels in gastric mucosa were measured through fluorescentquantitative PCR and immunohistochemistry.RESULTS H.pylori eradication rates were model control 0/10,Qingre Huashi Formula 3/10,triple therapy 7/10.Under the light microscope observation,model control group possessed massive H.pylori colonization and partial inflammatory cell infiltration.In triple therapy group,H.pylori colonization and inflammation was slight.Qingre Huashi Formula group had much local accumulation of H.pylori with no obvious inflammatory cell infiltration.The Il1b,Il8,Tnfa and Nfkb p65 mRNA expression levels of triple therapy and Qingre Huashi Formula groups were significantly higher than those of model control group.Immunohistochemistry results displayed no statistical significance among triple therapy,model control and Qingre Huashi Formula groups in the ratio of nucleus/cytoplasm optical density.CONCLUSION Qingre Huashi Formula has a limited eradication activity against H.pylori in infected mice with an obvious improvement of gastric mucosa inflammation.The Ill b,Il8,Tnfa and Nfkb p65 mRNA expression levels of Qingre Huashi Formula and triple therapy groups' markedly increasing with similar expression tendency is possibly related to the negative feedback effect induced by inhibition of protein levels,thus affects the occurance of inflammatory.