Simultaneous determination of six constituents in Tuiyin Mixture by HPLC / 中成药

ABSTRACT

AIM To establish an HPLC method for the simultaneous content determination of chlorogenic acid,loganin,paeoniflorin,baicalin,salvianolic acid B and paeonol in Tuiyin Mixture (Moutan Cortex,Paeoniae Radix Rubra,Lonicerae japonicae Caulis,etc.).METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic ZORBAX SB-Aq column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 235 nm.RESULTS Six constituents showed good linear relationships within their own ranges (r≥0.999 7),whose average recoveries were 97.1％-100.6％ with the RSDs of 1.35％-2.28％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Tuiyin Mixture.