Effect of fractional laser on the expression of SCF and c-kit in skin stem cells of guinea pig / 中华医学美学美容杂志

ABSTRACT

Objective To investigate the effect of fractional laser on the expression of stem cell factor (SCF) and c-kit receptor in body surface of guinea pig.Methods 20 guinea pigs were adopted.The back skin of each guinea pig was divided into four zonesA zone (the skin was not treated),B zone (the skin was treated by fractional laser at a low fluence20 mJ/cm2),C zone (the skin was treated at a medium fluence60 mJ/cm2),and D zone (the skin was treated at a high fluence120 mJ/cm2).The immunohistochemical method was used to detect the expression of SCF and c-kit in body surface of guinea pig.The results were quantified using an HPIAS-1000 system.Results One week later,the SCF expression level in B,C and D groups was increased obviously,which showed statistical difference compared with A group (P＜0.05);the c-kit expression level in C and D groups was increased obviously,compared with A group (P＜0.05).4 weeks later,the SCF expression level in C and D groups was higher than A and B groups;furthermore the level in D group was higher than C group (P＜0.05);the c-kit expression level in D group was higher than A and B groups (P＜0.05).Conclusions The fractional laser could generate the curative effect by increasing the expression of SCF and c-kit in body surface.