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MiR-192/-215 Regulates the Biological Functions of Gastric Cancer Cells by Targeting BIVM / 中山大学学报(医学科学版)
Article em Zh | WPRIM | ID: wpr-712958
Biblioteca responsável: WPRO
ABSTRACT
[Objective] To investigate the molecular mechanism of miR-192/-215 targeting BIVM in human gastric cancer.[Methods] First,BIVM was the target gene of miR-192/-215 screened by the target gene prediction in solico and gene microarrays.Real-time quantitative PCR verified the results of the microarrays.Then the double-luciferase reporter plasmids were constructed to test that BIVM was the target gene of miR-192/-215.Subsequently,BIVM-siRNA was transfected,to know the effects of BIVM-siRNA on proliferation and apoptosis of gastric cancer cells.8 nude mice were randomly divided into two groups:BIVM-siRNA experimental group and NSC-siRNA control group.Gastric cancer cells transfected with BIVM-SiRNA were implanted under the skin of nude mice to observe the effect of BIVM on the tumorigenicity of gastric cancer cells.[Results] BIVM was screened as miR-192/-215 target gene by gene microarrays and quantitative PCR.Double-luciferase reporting assays were performed to identify the BIVM as miR-192/-215 target gene.The cell proliferation assays showed that BIVM promoted the proliferation of gastric cancer cells (P<0.05).Test of flow cytometry showed that BIVM inhibited the apoptosis of gastric cancer cells (P<0.05).Mouse tumorigenesis test confirmed that BIVM could promote gastric cancer cells growth in vivo (P<0.05).[Conclusion] BIVM plays a role in the carcinogenesis of gastric cancer,and miR-192/-215 targeting BIVM promotes the development of gastric cancer.
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Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: Journal of Sun Yat-sen University(Medical Sciences) Ano de publicação: 2018 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: Journal of Sun Yat-sen University(Medical Sciences) Ano de publicação: 2018 Tipo de documento: Article