Electrophysiological Evidence for Functional Astrocytic P2X₃ Receptors in the Mouse Trigeminal Caudal Nucleus
Experimental Neurobiology
;
: 88-93, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-714118
ABSTRACT
Recently, we reported that astrocytes in the trigeminal caudal nucleus (Vc) of the brain stem express a purinergic receptor P2X₃, which is involved in the craniofacial pathologic pain. Although we observed protein expression of P2X₃ receptors (P2X₃ Rs) in the astrocyte of the Vc, it is still unclear that astrocyte has functional P2X₃Rs in Vc. To address this issue, we recorded asrtocytic P2X₃Rs by using whole cell voltage-clamp recording in the Vc of the GFAP-GFP mice, which was used as a guide to astrocytes with green fluorescence. While measuring voltage ramp-induced astrocytic membrane current, we found the amplitude of the current was increased when we applied P2-purinoreceptor agonist, α,β-meATP. This increase was blocked by co-application of A317491, P2X₃R antagonist. These results demonstrate that astrocytes in the Vc express functional P2X₃Rs, which might be critical in craniofacial pathologic pain.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Núcleo Inferior Caudal do Nervo Trigêmeo
/
Tronco Encefálico
/
Astrócitos
/
Eletrofisiologia
/
Fluorescência
/
Membranas
Limite:
Animais
Idioma:
Inglês
Revista:
Experimental Neurobiology
Ano de publicação:
2018
Tipo de documento:
Artigo
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