Divergent long-terminal-repeat retrotransposon families in the genome of Paragonimus westermani
The Korean Journal of Parasitology
;
: 221-231, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-7143
ABSTRACT
To gain information on retrotransposons in the genome of Paragonimus westermani, PCR was carried out with degenerate primers, specific to protease and reverse transcriptase (rt) genes of long-terminal-repeat (LTR) retrotransposons. The PCR products were cloned and sequenced, after which 12 different retrotransposon-related sequences were isolated from the trematode genome. These showed various degrees of identity to the polyprotein of divergent retrotransposon families. A phylogenetic analysis demonstrated that these sequences could be classified into three different families of LTR retrotransposons, namely, Xena, Bel, and Gypsy families. Of these, two mRNA transcripts were detected by reverse transcriptase-PCR, showing that these two elements preserved their mobile activities. The genomic distributions of these two sequences were found to be highly repetitive. These results suggest that there are diverse retrotransposons including the ancient Xena family in the genome of P. westermani, which may have been involved in the evolution of the host genome.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Paragonimus
/
Filogenia
/
Dados de Sequência Molecular
/
Alinhamento de Sequência
/
Sequência de Aminoácidos
/
DNA Polimerase Dirigida por RNA
/
Genoma
/
Clonagem Molecular
/
Análise de Sequência de DNA
/
Retroelementos
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2003
Tipo de documento:
Artigo
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