Viability of preserved Cryptosporidium baileyi oocysts
The Korean Journal of Parasitology
;
: 197-201, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-7146
ABSTRACT
The present study was undertaken to determine the viability and infectivity of oocysts of Cryptosporidium baileyi that had been stored from 1 to 40 months at 4 degrees C preserved in 2.5% potassium dichromate solution. Oocysts of C. baileyi were purified from the feces of experimentally infected chickens using discontinuous sucrose gradients. Subsequently, the purified oocysts were suspended in 2.5% potassium dichromate solution at a concentration of 1 x 10 (7) organism/ml, and their viabilities were assessed by nucleic acid staining, histologic examination, and infectivity to 2-day-old chickens. All chickens inoculated with oocysts that had been stored for 1-18 months developed patent infections, while chickens infected with older oocysts remained uninfected. Between 5.8% and 82.2% of the oocysts, stored at 4 degrees C in 2.5% potassium dichromate solution, were found to be viable, as determined by nucleic acid staining. Parasite colonization in the bursa of Fabricius was detected in the microvillus border of bursal epithelium. The finding that C. baileyi oocysts remain infective to chickens for at least 18 months offers important time-saving advantages to investigators who frequently require large numbers of oocysts.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Compostos Orgânicos
/
Dicromato de Potássio
/
Doenças das Aves Domésticas
/
Preservação Biológica
/
Coloração e Rotulagem
/
Bolsa de Fabricius
/
Galinhas
/
Criptosporidiose
/
Cryptosporidium
/
Oocistos
Limite:
Animais
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2003
Tipo de documento:
Artigo
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