TRPV1 Blocking Alleviates Airway Inflammation and Remodeling in a Chronic Asthma Murine Model
Allergy, Asthma & Immunology Research
;
: 216-224, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-714725
ABSTRACT
PURPOSE:
Asthma is a chronic inflammatory airway disease characterized by airway hyperresponsiveness (AHR), inflammation, and remodeling. There is emerging interest in the involvement of the transient receptor potential vanilloid 1 (TRPV1) channel in the pathophysiology of asthma. This study examined whether TRPV1 antagonism alleviates asthma features in a murine model of chronic asthma.METHODS:
BALB/c mice were sensitized to and challenged by ovalbumin to develop chronic asthma. Capsazepine (TRPV1 antagonist) or TRPV1 small interfering RNA (siRNA) was administered in the treatment group to evaluate the effect of TPV1 antagonism on AHR, airway inflammation, and remodeling.RESULTS:
The mice displayed increased AHR, airway inflammation, and remodeling. Treatment with capsazepine or TRPV1 siRNA reduced AHR to methacholine and airway inflammation. Type 2 T helper (Th2) cytokines (interleukin [IL]-4, IL-5, and IL-13) were reduced and epithelial cell-derived cytokines (thymic stromal lymphopoietin [TSLP], IL-33, and IL-25), which regulate Th2 cytokine-associated inflammation, were also reduced. Airway remodeling characterized by goblet cell hyperplasia, increased α-smooth muscle action, and collagen deposition was also alleviated by both treatments.CONCLUSIONS:
Treatment directed at TRPV1 significantly alleviated AHR, airway inflammation, and remodeling in a chronic asthma murine model. The TRPV1 receptor can be a potential drug target for chronic bronchial asthma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Asma
/
Ovalbumina
/
Citocinas
/
Cloreto de Metacolina
/
Colágeno
/
Interleucina-5
/
Células Caliciformes
/
RNA Interferente Pequeno
/
Remodelação das Vias Aéreas
/
Interleucina-33
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Allergy, Asthma & Immunology Research
Ano de publicação:
2018
Tipo de documento:
Artigo
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